POLYPHENOLIC WEALTHY MORINGA LEAVES EXTRACTS AS ANTICOXSACKIE B VIRUSES (COX-BV)

Saif, Naglaa A.; Othman, B. A.; Mohamed, A. F.; El-Dougdoug, Kh. A.

doi:10.21608/ajs.2019.43350

POLYPHENOLIC WEALTHY MORINGA LEAVES EXTRACTS AS ANTICOXSACKIE B VIRUSES (COX-BV)

Document Type : Original Article

Authors

¹ General Authority for Health Insurance Hospital, Sednawy, Cairo, Egypt.

² Agric. Microbiology Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadayek Shobra 11241, Cairo, Egypt

³ Holding Company for Biological product and vaccines, Giza, Egypt.

10.21608/ajs.2019.43350

Abstract

The antiviral role of Moringaoleifera leaves extracts ((chloroform (CL), Ethyl acetate (E.A), methanol 80% (M) and n.butanol (n.b) to control Coxsackie B (COX-BV) viral infection was monitored in vitro compared with human interferon alpha (IFNᾰ2a) in order to evaluate the antiviral activity of moringa leaves extracts. The phenolic compound was given higher conc. in E.A. extracts. Cytotoxicity was determined for Moringa leaves extracts on viability of HEP2 cell using MTT assay. The 98μg/ml concentrations for four extracts were considerably non-toxic for HEP2 cell line culture. Antiviral activity in Moringa extracts may be attributed to phenolic compounds and / or as a result of stimulation of COX-BV-sensitive HEP2 cells to express MX protein. Antiviral activity of Moringa leaves extracts compared with IFNᾰ-2a to cell lines against COX-BV viral infection was determined by assessment of the COX-BV virus infectivity titer declining rate and relative residual living cell count using End Point Assay, using three ways pretreatment, co-treatment and post-treatment cell line treated with Moringaextracts. It was found that the extract acetyl acetate was better as an MX gene expression level compared with its level in control cells. It was concluded that Moringaleaves extracts have antiviral activity against COX-BV.

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