Phylogeny of Three Species Belonging to Genus Potyvirus Infecting Solanum tuberosum Based on Biological and Molecular Variability

El-Turkey, Aya Hussin; El-Attar, Ahmed Kamal; Othman, Badawi Abdel Salam; El Dougdoug, K. A.

doi:10.21608/ajs.2025.319743.1583

Phylogeny of Three Species Belonging to Genus Potyvirus Infecting Solanum tuberosum Based on Biological and Molecular Variability

Document Type : Original Article

Authors

¹ Virus Research Dept, Plant Pathology Research Institute, ARC, Giza 12619, Egypt

² Agricultural Microbiology Dept, Fac of Agric, Ain Shams Univ, P.O. Box 68, Hadayek Shubra 11241, Cairo, Egypt

10.21608/ajs.2025.319743.1583

Abstract

Potato virus Y exhibits diverse strains and complexities circulat-ing within potato fields, leading to yield deterioration. This study examined potato virus Y (PVY) isolates for their varied symptom expressions on po-tato plants. The infected potato plants were categorized into three distinct phenotypic expressions: venial necrosis (PVY-VN), yellow mosaic (PVY-YM), and mottling pattern (PVY-MP). They showed various biological in-dicators, including disease density index, pathogenicity, virus concentration via DAS-ELISA, and molecular variability through HC-PRO silencing gene accumulation via qRT-PCR. The phylogeny of the HC-PRO silencing gene revealed variations in similarity and genetic distance, corresponding to dif-ferences in the 207 nucleotides. These variations were observed among the three PVY isolates and between them and other PVY strains registered in the GenBank database. They were registered in GenBank under the acces-sion numbers #OR804503, #PQ015611, and #PQ015612, corresponding to PVY-VN, PVY-YM, and PVY-MP, respectively. Based on the phylogeny, the three isolates belong to the NTN strain and exhibit a sensitivity range of 99% to 100% with AJ585342_United-Kingdom-strain_NTN_sequence and KY092173_Poland_strain_sequence.

Keywords