Isolation and Identification of Bacteria Producing Indole From Rhizospheric Plant

Saad, Dina M; El- Demerdash, Mohamed E; Abdellatif, Yasmin MR; Hassan, Enas Abd El- Tawab

doi:10.21608/ajs.2024.305171.1577

Isolation and Identification of Bacteria Producing Indole From Rhizospheric Plant

Document Type : Short Communication

Authors

¹ Agricultural Microbiology Dept, Fac of Agric, Ain Shames Univ, P.O. Box 68, Hadayek Shubra 11241, Cairo, Egypt

² Agricultural Botany Dept, Fac of Agric, Ain Shames Univ, P.O. Box 68, Hadayek Shubra 11241, Cairo, Egypt

10.21608/ajs.2024.305171.1577

Abstract

Forty-six bacterial isolates were obtained from seven rhizosphere samples using nutrient agar and glucose agar media. These isolates were morphologically categorized into long rods, short rods, and cocci, comprising 52%, 17%, and 31% of the total count, respectively. The collected isolates from the rhizosphere were assessed for their capability to produce indole-3-acetic acid (IAA). The initial qualitative screening revealed diverse abilities among isolates for IAA production, with notable variations in productivity levels. Quantitative analysis of the top ten isolates revealed that cocci-shaped isolates produced the highest IAA levels (4.80 to 6.30 mg/100 mL), with the SS1 isolate achieving the maximum value (6.30 mg/100 mL). Genetic identification of the highest IAA producer (SS1) through 16S rRNA gene sequencing showed that it belongs to the genus Kocuria. Phylogenomic analysis confirmed a high similarity to Kocuria rosea, with 98% identity.

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