Optimizing CuSO4 and CoCl2 for Superior Somatic Embryos Regeneration of Date Palm

Abdelmotagaly, Sara Mohamed; Abdellatif, Yasmin; Zein Eldin, Amal F.M.; Abd Elbar, Ola Hussein; Ibrahim, Ibrahim Seif Eldin

doi:https://doi.org/10.21608/AJS.2024.270961.1557

Optimizing CuSO4 and CoCl2 for Superior Somatic Embryos Regeneration of Date Palm

Document Type : Original Article

Authors

¹ Agricultural Botany Dept, Fac of Agric, Ain Shams Univ, P.O. Box 68, Hadayek Shubra 11241, Cairo, Egypt

² The Central Laboratory for Date Palm Researches and Development, Agricultural Research Center, Giza 12619, Egypt

https://doi.org/10.21608/AJS.2024.270961.1557

Abstract

The purpose of the research was to improve the morpho-anatomical and physiological features of somatic embryos (SEs) of date palms in tissue culture media that have the potential to produce green and healthy plantlets. Copper sulfate (CuSO₄) and cobalt chloride (CoCl₂) were supplemented to date palm maturation medium at various concentrations, separately or combined. Adding CoCl₂ to the media of maturation produced the greatest relative growth and differentiation percentages of date palm embryogenic calli, particularly at 0.52 mgL^-1. In comparison to the control, when combined treatments were administered alone, the quantity of the produced somatic embryos rose dramatically (17/jar). Total sugars and free amino acids were the highest in SEs grown on a maturation medium withCoCl₂at 0.26 mgL^-¹. Moreover, total phenols and indoles were picked significantly at their higher levels in the generated SEs that were cultured on a medium enhanced with 0.52 mgL^-1. When the maturation medium was administered along with both as a treatment, the antioxidant enzyme, ascorbate peroxidase, reached its maximum activity. Whereas, adding 0.52 mgL^-1 of CoCl₂ to the culture medium enhanced the activities up to of highest levels of ascorbate peroxidase, polyphenol oxidase and phenylalanine ammonia-lyase.

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