ISOLATION AND MOLECULAR IDENTIFICATION OF INDIGENOUS BACTERIAL ISOLATES ABLE TO DEGRADE ORGANOPHOSPHATES

El-sayed, Ghada M.; Ibrahim,, S. A.; Abosereh, Nivien A.; Abd El-Razik, A. B.; Hafez, Fatma M.; Hammad, M. A.

doi:10.21608/ajs.2018.35617

ISOLATION AND MOLECULAR IDENTIFICATION OF INDIGENOUS BACTERIAL ISOLATES ABLE TO DEGRADE ORGANOPHOSPHATES

Document Type : Original Article

Authors

¹ Microbial Genetics Dept., National Research Centre., P.O. Box 12622, 33 El-Bohouth St., Dokki, Giza, Egypt

² Genetic. Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadayek Shubra 11241, Cairo, Egypt

³ Plant Protection Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadayek Shobra 11241, Cairo, Egypt

10.21608/ajs.2018.35617

Abstract

The wide and indiscriminate use of pesticides for pest control in agriculture has inflicted serious harm and problems to humans as well as to the biodiversity. Microbial degradation of pesticides in contaminated soils has been considered advantageous to decontaminate areas that have been polluted by pesticides. Chlorpyrifos and diazinon were the most persistent residues in Egyptian soils. Four bacterial isolates were isolated from organophosphorus insecticides contaminated soils and genetically identified based on DNA sequence of 16s rDNA gene, Cronobacter muytjensii GH10, Achromobacter xylosoxidans GH9OP, Pseudomonas aeruginosa GH2NO8 and Pseudomonas putida GH4SNO/P were able to degrade 92.59%, 97.75%, 91.82%, and 90.78% of diazinon (600mg/l) as compared with 16.99% in control and 93.43%, 78.51%, 93.18% and 95.36% of chlorpyrifos (480mg/l) as compared with 4.28%, in control, respectively after 20 days of incubation.

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