Document Type : Original Article
Authors
1
The Arab Center for the Studies of Arid Zones and Dry Lands (ACSAD), Damascus, Syia
2
Genetics Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadayek Shobra 11241, Cairo, Egypt
3
Genetics Dept., Fac. of Agric., Cairo Univ., Giza, Egypt
4
2- Genetics Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadayek Shobra 11241, Cairo, Egypt
Abstract
In the present study, molecular fingerprinting
among selected ten seeded type date palm trees
growing at Al Dakhlah Oasis, New Valley Governorate
in Egypt was carried out, in this approach
10 RAPD and 10 ISSR primers were used. In
RAPD markers, primers 1, 3, 4, and 6 showed the
highest level of polymorphism with number of polymorphic
amplicons of 100%. While primers 7 and
8 resulted in the lowest number of polymorphic
bands with polymorphism level of 62% and 63%,
respectively. The similarity between Acsad-Dakhla
6 and 7 (0.76%) was observed as maximum similarity.
While, Acsad-Dakhla 5 exhibited a minimum
degree of similarity with all the seeded type date
palm trees ranging from 33% to 49%. According to
ISSR, highest polymorphism was generated by
primers 8 and 9 (100%). While primers 4 and 2
produced the lowest polymorphism 50% and
55.6%, respectively. The highest similarity was
observed between Acsad-Dakhla 8 and Acsad-
Dakhla 9. Acsad-Dakhla 5 and Acsad-Dakhla 7
showed close relationship but Acsad-Dakhla 10
was at distant from all the seeded type date palm
trees and did not lie in any subcluster. In combined
analysis, the similarity between Acsad-Dakhla 6
and 7 were similar to Acsad-Dakhla 9 and 10
showed maximum similarity (85%). While, Acsad-
Dakhla 2 and 5 exhibited a minimum degree of
similarity with all the seeded type date palm trees
(47%). A high genetic diversity among selected ten
seeded type date palm trees was present.
Keywords
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