THE siRNA EFFICACY OF SOLUBLE ACID INVERTASE DOWN-REGULATION IN SUGARCANE (SACCHARUM SPP.)

Document Type : Original Article

Authors

1 Sugar Crop Research Institue (SCRI), Agriculture Research Center (ARC), Giza, Egypt

2 Genetics Dept., Fac. of Agric., Ain-Shams Univ., P.O. Box 68 Hadayek Shoubra 11241, Cairo, Egypt

3 Genetics Dept., Fac. of Agric., Beni-suef Univ., Beni-suef, Egypt

Abstract

Sugarcane (Saccharum sp. hybrids) is a C4 grass used as a major source of sucrose. Invertase enzymes hydrolyse sucrose into hexose sugars reducing the production markedly. Soluble acid invertase role is always a case of discussion for having a major or minor role in the breakdown process in sink tissues. Bio-deterioration is another serious problem accomplishes the sucrose production, the delay between harvest and milling of sugarcane cause enormous depreciation in cane tonnage as well as sugar recovery. Beside another many factors, it was improved that both neutral and acid invertase present in cane stalk and both have tendency to increase after harvest. In the present study, sugarcane cultivar G.99/103, Saccharum
 officinarium, was used to establish Inplanta transformation experiment for down-regulation of soluble acid invertase gene using siRNA application. The transgenic plants were examined chemically and genetically to estimate the percentage of silencing and its impact on the sucrose content. The enzyme activity showed reduction compared to control in most transgenic plants and consequently the decrease in expression level of soluble acid invertase increase the Brix value significantly in some of the transgenic plants. 
 

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