Ahmed, A., Selim, S., Zayed, M., Abd El-Fattah, D. (2022). Isolation and Identification of Some Egyptian Ectomycorrhizal Sporocarps. Arab Universities Journal of Agricultural Sciences, 30(1), 55-59. doi: 10.21608/ajs.2022.123225.1470
Aya Ahmed; Sawky Selim; Mona Zayed; Dalia Abd El-Fattah. "Isolation and Identification of Some Egyptian Ectomycorrhizal Sporocarps". Arab Universities Journal of Agricultural Sciences, 30, 1, 2022, 55-59. doi: 10.21608/ajs.2022.123225.1470
Ahmed, A., Selim, S., Zayed, M., Abd El-Fattah, D. (2022). 'Isolation and Identification of Some Egyptian Ectomycorrhizal Sporocarps', Arab Universities Journal of Agricultural Sciences, 30(1), pp. 55-59. doi: 10.21608/ajs.2022.123225.1470
Ahmed, A., Selim, S., Zayed, M., Abd El-Fattah, D. Isolation and Identification of Some Egyptian Ectomycorrhizal Sporocarps. Arab Universities Journal of Agricultural Sciences, 2022; 30(1): 55-59. doi: 10.21608/ajs.2022.123225.1470
Isolation and Identification of Some Egyptian Ectomycorrhizal Sporocarps
1Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
2Central Laboratory for Agricultural Climate, Agricultural Research Center, Giza, Egypt
Abstract
In contrast to the global countries, ectomycorrhizae members are not common in Egypt, however, they are very important in the afforestation of poor lands. Their occurrence in Pinus sp., Clerodendrum sp., and Eucalyptus sp. may help to explain why these trees are the most extensive dominant around the world, especially in Egypt. The identification and characterization of numerous ectomycorrhizal fungi often involve largely morphological descriptions of sporocarps alone, which consequently, in some instances, rise argument about the accuracy of these studies. The present work was achieved to isolate, identify, and characterize three ectomycorrhizal fungi from sporocarps combining morphological and molecular techniques. Morphological characteristics of tested species were assessed and compared to standard taxonomic literature. ITS-rDNA was utilized for molecular analysis using the universal fungal primers ITS1. Identification of these species was confirmed by comparing the sequences of amplified genomes of these species with respective species sequences in GenBank, followed by blast analysis.
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