SUSCEPTIBILITY OF DESERT LOCUST, SCHISTOCERCA GREGARIA (ORTHOPTERA: ACRIDIDAE) TO BACILLUS CEREUS ISOLATED FROM EGYPT

Document Type : Original Article

Authors

1 Locust and Grasshoppers Research Dept., Plant Protection Research Institute, Agric.

2 Plant Protection Dept., Fac. of Agric., Ain Shams Univ., Cairo, Egypt.

3 Food Sci. Dept., Fac. of Agric., Ain Shams Univ., Cairo, Egypt.

4 Locust and Grasshoppers Research Dept., Plant Protection Research Institute, Agric. Research Centre. Dokki, Giza, Egypt

5 Microbiological Resources Centre (MIRCEN), Fac. of Agric., Ain Shams Univ., Cairo, Egypt

Abstract

Examination was done at preliminary bracketing bioassay on one old 4th nymphal instar of desert locust. Results showed that two isolates, namely NDL1 and NDL2 were having highly potentiality as entomopathogenic bioagents. Thirty isolates were isolated from dead/ infected nymphs of desert locust occurred in raring cages at Department of Locust and Grasshoppers Research, Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt. Molecular identification of isolated bacteria was done using universal primers of 16s rRNA, followed by DNA sequencing. Nucleotides were blasted at (https://www.ncbi. nlm.nih.gov /genbank/) to recognize that NDL1 and NDL2 isolates were two different isolates of Bacillus cereus with a high similarity (100%).  Susceptibility of 4th nymphal instar of Schistocerca gregaria (Forskal) to the isolated B. cereus was determined using two bioassay procedures, Leaf-dip and per os. The insecticidal activity of both isolates against locust nymph in leaf dipping showed that NDL2 was more efficient than NDL1. However, the opposite trend was observed in using per os.  Both Isolates have the potential to be a successful biocidal agent to control desert locust.

Keywords

Arab Universities Journal of Agricultural Sciences
Volume 26, Issue 2 - Serial Number 1
Agric. Economic No. 32 pp. 415 – 434
September 2018
Pages 725-734

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