ISOLATION, PURIFICATION AND IDENTIFICATION OF SOME MICROORGANISMS PRODUCE PLANT GROWTH PROMOTING SUBSTANCES (METHYLOTROPHIC BACTERIA)

Document Type : Original Article

Authors

1 Department of Agricultural Microbiology, Soils, Water & Environment Research Institute, Agricultural Research Center, Giza, Egypt.

2 Department of Agricultural Microbiology, Soils, Water & Environment Research Institute, Agricultural Research Center, Giza, Egypt

3 Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams Uni-versity, Shoubra El-Kheima, Cairo, Egypt.

Abstract

Recently, the potential economical importance of the methylotrophic bacteria encouraged the isolation of this group. In the present study five Egyptian isolates were obtained from green leaves surface of legume plants named PPFM.C (ChickPea), PPFM.Ph (Common bean), PPFM.F (Faba bean), PPFM.P (Peanut) and PPFM.S (Soybean) ,to study their general characters which belonging to  methylotrophic bacteria. Morphological studies indicate that all isolates were short rods, gram negative and motile. All Physiological studies to the isolates gave the same results except PPFM.F which could not grow in peptone medium. All isolates were sensitive to Kanamycin but they were resistant to Erythromycin. There was a great range in the ability of the isolates to grow on different sodium chloride concentrations indicating that PPFM.Ph grew well in 5 % sodium chloride, and they were able to excrete and produce cytokinin. Molecular biology studies indicated that there was a great similarity between PPFM.C and PPFM.Ph (99.34%). Identification was carried out to the5 isolates, PPFM.F may be related to Methylobacterium mesophilicum, PPFM.P may be related to M. fujisawaense and PPFM.Ph, PPFM.C and PPFM.S were related to M. radiotolerans. 

Keywords

Arab Universities Journal of Agricultural Sciences
Volume 13, Issue 3
September 2005
Pages 717-729

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