MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF ECTOMYCORRHIZAL FUNGI ISOLATED FROM EGYPTIAN ENVIRONMENT

Zayed, Mona; Selim, Sh; Eweeda, Wedad E; Ali, M K; Hazem, A

doi:10.21608/ajs.2007.14854

MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF ECTOMYCORRHIZAL FUNGI ISOLATED FROM EGYPTIAN ENVIRONMENT

Document Type : Original Article

Authors

¹ Microbiology Dept.. Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo 2-Unit of Biofertiliz., Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo

² 1-Microbiology Dept.. Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo 2-Unit of Biofertiliz., Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo

³ 1. Microbiology Dept.. Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo 2. Unit of Biofertiliz., Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo

⁴ Plant Pathology Dept., Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo

⁵ Microbiology Dept.. Fac. Agric., Ain Shams Univ., Shobra El-Kheima, Cairo

10.21608/ajs.2007.14854

Abstract

In a trial to isolate and identify ectomycorrhi-zal fungi for the first time in Egypt 13 sporocarpes associated with rang of plants grown on Egyptian soils were collected, to be used for this purpose. Fungal isolates were obtained from collected spo-rocarpes and tested for ectomycorrhiza formation with Bauhinia sp. and Pinus seedlings. The paper sandwich technique was used for simultaneous inoculation of root apices Bauhinia to produce synchronously developing ectomycorrhizas. Typi-cal ectomycorrhizal roots were obtained within 14 days after inoculation with the collected strains. The isolates tested for ectomycorrhiza formation with Pinus sp. to ensure their identity by for-mation of distinct root characteristics on this host. Root colonization levels varied markedly among the tested fungal strains with respect to growth enhancement and NPK uptake of Pinus shoots and roots.

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